Human BAFF/CD257 (B-Cell Activating Factor) CLIA Kit
The Human BAFF (B-cell Activating Factor) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of BAFF levels in human samples including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it a valuable tool for researchers in various fields.BAFF is a key cytokine involved in the activation and survival of B cells, playing a crucial role in the regulation of the immune response.
Dysregulation of BAFF has been associated with autoimmune diseases, such as lupus and rheumatoid arthritis, making it an important biomarker for studying these conditions and developing targeted therapies.Overall, the Human BAFF CLIA Kit offers researchers a powerful tool for investigating the role of BAFF in various physiological and pathological processes, with potential implications for the development of new diagnostic and therapeutic approaches.
Product Name:
Human BAFF/CD257 (B-Cell Activating Factor) CLIA Kit
SKU:
HUES00009
Target:
Human BAFF/CD257 (B-Cell Activating Factor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human BAFF/CD257. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BAFF/CD257 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BAFF/CD257, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human BAFF/CD257. You can calculate the concentration of Human BAFF/CD257 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
87-97
88-103
Average (%)
90
92
95
1:4
Range (%)
88-99
93-110
91-103
Average (%)
93
100
97
1:8
Range (%)
89-105
93-108
86-100
Average (%)
96
101
93
1:16
Range (%)
101-114
89-104
90-102
Average (%)
108
95
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-114
105
EDTA plasma (n=5)
96-109
104
Cell culture media (n=5)
94-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.85
120.32
380.95
44.26
112.8
385.46
Standard deviation
5.42
11.6
37.3
3.66
9.85
37.58
C V (%)
11.1
9.64
9.79
8.27
8.73
9.75
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human BAFF/CD257 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human BAFF/CD257 in samples. No significant cross-reactivity or interference between Human BAFF/CD257 and analogues was observed.
