Human BAG3 (Bcl2 Associated Athanogene 3) CLIA Kit
The Human Bag3 (BCL2-Associated Athanogene 3) CLIA Kit is a cutting-edge diagnostic tool designed for the precise measurement of Bag3 levels in human biological samples such as serum, plasma, and cell culture supernatants. This advanced kit offers exceptional sensitivity and specificity, ensuring reliable and consistent results for a variety of research applications.Bag3, also known as BCL2-Associated Athanogene 3, is a critical protein involved in cellular stress responses and regulating cell survival. Dysregulation of Bag3 has been linked to various disease states, including cancer, cardiovascular disorders, and neurodegenerative conditions, making it a valuable biomarker for understanding these diseases and developing potential treatment strategies.
With its high-performance capabilities, the Human Bag3 CLIA Kit is an indispensable tool for researchers and clinicians seeking to explore the role of Bag3 in disease pathogenesis and therapeutic interventions. Stay ahead in your research with this state-of-the-art diagnostic kit from AssayGenie.
Product Name:
Human BAG3 (Bcl2 Associated Athanogene 3) CLIA Kit
SKU:
HUES00300
Target:
Human BAG3 (Bcl2 Associated Athanogene 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human BAG3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BAG3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BAG3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human BAG3. You can calculate the concentration of Human BAG3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
94-108
101-118
Average (%)
91
102
108
1:4
Range (%)
86-100
101-120
91-106
Average (%)
92
109
98
1:8
Range (%)
91-104
90-102
92-105
Average (%)
98
96
99
1:16
Range (%)
90-105
90-105
98-112
Average (%)
97
97
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-108
99
EDTA plasma (n=5)
88-103
95
Cell culture media (n=5)
99-113
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.0
180.6
975.47
93.66
179.48
1051.19
Standard deviation
8.06
13.17
69.26
8.8
17.01
79.79
C V (%)
8.48
7.29
7.1
9.4
9.48
7.59
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human BAG3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human BAG3 in samples. No significant cross-reactivity or interference between Human BAG3 and analogues was observed.
