The Human BCAN (Brevican) CLIA Kit is specifically designed for the quantitative detection of brevican levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and dependable results for a variety of research applications.Brevican is a critical component of the extracellular matrix in the central nervous system, where it plays a role in regulating synaptic plasticity and cell adhesion. Dysregulation of brevican has been implicated in diseases such as gliomas and neuroinflammatory disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
Overall, the Human BCAN CLIA Kit provides researchers with a powerful tool for investigating the role of brevican in various physiological and pathological processes, ultimately advancing our understanding of neurological disorders and potentially leading to new treatment strategies.
Product Name:
Human BCAN (Brevican) CLIA Kit
SKU:
HUES00403
Target:
Human BCAN (Brevican)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human BCAN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BCAN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BCAN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human BCAN. You can calculate the concentration of Human BCAN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-120
97-111
87-103
Average (%)
110
105
94
1:4
Range (%)
88-103
87-102
98-112
Average (%)
95
94
104
1:8
Range (%)
101-119
101-115
98-112
Average (%)
109
109
106
1:16
Range (%)
93-108
95-107
97-113
Average (%)
99
102
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-114
108
EDTA plasma (n=5)
98-112
105
Cell culture media (n=5)
91-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
202.28
385.1
1447.18
201.64
346.96
1539.55
Standard deviation
19.54
40.78
142.84
18.21
36.85
136.4
C V (%)
9.66
10.59
9.87
9.03
10.62
8.86
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human BCAN concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human BCAN in samples. No significant cross-reactivity or interference between Human BCAN and analogues was observed.
