Human BCAR1 (Breast Cancer Anti Estrogen Resistance 1) CLIA Kit
The Human BCAR1 (Breast Cancer Anti-Estrogen Resistance 1) CLIA Kit is specifically designed for the quantification of BCAR1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high accuracy and precision, ensuring dependable and consistent results for various research applications.BCAR1 is a key protein associated with anti-estrogen resistance in breast cancer, playing a crucial role in tumor progression and resistance to hormone therapy. By measuring BCAR1 levels, researchers can gain valuable insights into the mechanisms of breast cancer development and identify potential targets for therapeutic interventions.
With its reliable performance and sensitivity, the Human BCAR1 CLIA Kit is a valuable tool for studying breast cancer and exploring new treatment options for patients with anti-estrogen-resistant tumors. Trust in this kit to provide accurate and reproducible data for advancing our understanding of breast cancer biology and improving patient outcomes.
Product Name:
Human BCAR1 (Breast Cancer Anti Estrogen Resistance 1) CLIA Kit
SKU:
HUES01230
Target:
Human BCAR1 (Breast Cancer Anti Estrogen Resistance 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human BCAR1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BCAR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BCAR1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human BCAR1. You can calculate the concentration of Human BCAR1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-110
97-111
85-100
Average (%)
102
103
92
1:4
Range (%)
93-106
102-116
101-113
Average (%)
98
110
107
1:8
Range (%)
99-111
103-119
94-106
Average (%)
105
109
100
1:16
Range (%)
95-110
97-112
89-100
Average (%)
101
104
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
101
EDTA plasma (n=5)
99-113
106
Cell culture media (n=5)
95-106
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.9
284.94
882.64
90.87
270.63
890.03
Standard deviation
8.21
23.19
94.8
10.87
28.04
97.9
C V (%)
8.56
8.14
10.74
11.96
10.36
11.0
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human BCAR1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human BCAR1 in samples. No significant cross-reactivity or interference between Human BCAR1 and analogues was observed.
