The Human BCL-2 (B-Cell Leukemia/Lymphoma 2) CLIA Kit is a cutting-edge diagnostic tool designed for the precise measurement of BCL-2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.BCL-2 is a critical protein that regulates apoptosis and cell survival, playing a key role in various diseases including leukemia, lymphoma, and other cancers.
By measuring BCL-2 levels, researchers can gain valuable insights into disease progression and potential treatment strategies.Overall, the Human BCL-2 CLIA Kit is a valuable asset for researchers seeking to understand the role of BCL-2 in disease pathogenesis and explore potential therapeutic interventions. Its reliability and precision make it an indispensable tool in the field of biomedical research.
Product Name:
Human Bcl-2 (B-cell Leukemia/Lymphoma 2) CLIA Kit
SKU:
HUES00109
Target:
Human Bcl-2 (B-cell Leukemia/Lymphoma 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Bcl-2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Bcl-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Bcl-2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human Bcl-2. You can calculate the concentration of Human Bcl-2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-106
91-109
99-112
Average (%)
97
99
106
1:4
Range (%)
91-107
89-99
98-112
Average (%)
98
94
104
1:8
Range (%)
89-106
95-109
96-111
Average (%)
97
101
103
1:16
Range (%)
97-114
89-104
85-96
Average (%)
104
95
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
92
EDTA plasma (n=5)
96-111
104
Cell culture media (n=5)
93-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
214.89
456.63
1932.39
213.61
468.8
1808.51
Standard deviation
20.93
44.89
182.61
20.74
50.86
184.65
C V (%)
9.74
9.83
9.45
9.71
10.85
10.21
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human Bcl-2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Bcl-2 in samples. No significant cross-reactivity or interference between Human Bcl-2 and analogues was observed.
