Description
| Product SKU: | SBRS0008 |
| Size: | 96T |
| Application: | This ELISA kit is designed to detect human, mouse, and rat Beta-defensin 1. |
| Uniprot: | P60022 |
| Gene ID: | 1672 |
| Synonyms: | Beta-defensin 1 (BD-1) (hBD-1) (Defensin, beta 1) |
| Target Species: | Human, Mouse, Rat |
| Compatible Sample Types: | Cell Culture Supernatants, Serum |
| Design Principle: | Competition-based |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Range: | 0.1-1,000 ng/ml |
| Sensitivity: | 0.9 ng/ml |
| Recommended Dilution: | Human: 2x, Mouse: 2x, Rat: 2x |
| Storage/Stability: | Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2 to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge. |
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- Technical Manual
Other materials and equipment required:
The Assay Genie Human Beta-defensin 1 PharmaGenie ELISA Kit (SBRS0008) will require other equipment and materials to carry out the assay. Please see list below for further details.
- Distilled or deionized water
- Precision pipettes to deliver 2 ul to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
- Prepare all reagents, samples and standards as instructed.
- Add 100 ul detection antibody to each well.
- Incubate 1.5 h at RT or O/N at 4°C.
- Add 100 ul standard or sample to each well.
- Incubate 2.5 h at RT.
- Add 100 ul prepared streptavidin solution.
- Incubate 45 min at RT.
- Add 100 ul TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 ul Stop Solution to each well.
- Read plate at 450 nm immediately.
 | Standard Curve for Human Beta-defensin 1 PharmaGenie ELISA Kit (SBES0008) |
Beta-defensin 1 (BD-1) is a 36 amino acid peptide that has bactericidal activity in humans. It is encoded by the DEFB1 gene, and belongs to a family of microbicidal and cytotoxic peptides made by neutrophils. BD-1 is believed to function in the antimicrobial defense of the urogenital and respiratory tracts.
