The Human Beta TG (Beta Thromboglobulin) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of beta thromboglobulin levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it suitable for a variety of research applications.Beta thromboglobulin is a critical protein involved in platelet activation and aggregation, playing a crucial role in the formation of blood clots. Abnormal levels of beta thromboglobulin have been associated with various diseases, including thrombosis, cardiovascular diseases, and immune disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
With the Human Beta TG CLIA Kit, researchers can confidently assess beta thromboglobulin levels in biological samples, enabling further understanding of its role in disease pathogenesis and progression. This kit is a valuable tool for both basic research and clinical studies focused on identifying new diagnostic and therapeutic targets related to beta thromboglobulin regulation.
Product Name:
Human beta-TG (beta-Thromboglobulin) CLIA Kit
SKU:
HUES00595
Target:
Human beta-TG (beta-Thromboglobulin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human β-TG. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human β-TG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human β-TG, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human β-TG. You can calculate the concentration of Human β-TG in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-101
90-106
88-103
Average (%)
95
98
94
1:4
Range (%)
91-103
85-99
93-106
Average (%)
97
92
98
1:8
Range (%)
102-115
87-100
85-99
Average (%)
109
95
92
1:16
Range (%)
94-107
100-114
94-111
Average (%)
101
107
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-116
106
EDTA plasma (n=5)
87-100
94
Cell culture media (n=5)
92-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.94
137.59
363.67
48.57
131.95
369.98
Standard deviation
6.19
11.23
38.04
5.34
11.36
23.27
C V (%)
11.92
8.16
10.46
10.99
8.61
6.29
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human β-TG concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human β-TG in samples. No significant cross-reactivity or interference between Human β-TG and analogues was observed.
