The Human BGN (Biglycan) CLIA Kit is specifically designed for the precise detection of biglycan levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and accurate results for a variety of research purposes.Biglycan is a key component in the extracellular matrix and has been implicated in various biological processes, including inflammation, fibrosis, and tissue repair.
Abnormal levels of biglycan have been linked to conditions such as arthritis, cardiovascular diseases, and cancer, underscoring its importance as a potential biomarker for disease progression and therapeutic interventions.With the Human BGN CLIA Kit, researchers can confidently study the role of biglycan in health and disease, paving the way for new insights and treatment strategies in medicine and biotechnology.
Product Name:
Human BGN (Biglycan) CLIA Kit
SKU:
HUES01040
Target:
Human BGN (Biglycan)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human BGN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BGN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BGN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human BGN. You can calculate the concentration of Human BGN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
95-109
87-102
Average (%)
92
100
94
1:4
Range (%)
90-103
92-108
98-115
Average (%)
96
100
105
1:8
Range (%)
87-100
95-108
94-109
Average (%)
92
100
102
1:16
Range (%)
88-101
94-108
97-112
Average (%)
95
100
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-115
107
EDTA plasma (n=5)
101-116
108
Cell culture media (n=5)
100-117
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
210.38
635.83
1869.21
190.72
672.44
2009.47
Standard deviation
17.31
50.68
157.01
16.52
68.93
177.84
C V (%)
8.23
7.97
8.4
8.66
10.25
8.85
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human BGN concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human BGN in samples. No significant cross-reactivity or interference between Human BGN and analogues was observed.
