Human BMP-1 (Bone Morphogenetic Protein 1) ELISA Kit
The Human BMP-1 (Bone Morphogenetic Protein 1) ELISA Kit is specifically developed for the quantitative measurement of Bone Morphogenetic Protein 1 (BMP-1) levels in various human biological samples. BMP-1 is a key protein involved in bone formation and repair, as well as in the regulation of other crucial cellular processes and signaling pathways. BMP-1 plays a pivotal role in skeletal development, tissue regeneration, and extracellular matrix maintenance. Accurate quantification of BMP-1 is essential for studying its functions in bone metabolism, developmental processes, and tissue homeostasis.
This ELISA kit provides exceptional sensitivity and specificity, ensuring precise and reproducible results. Manufactured under strict quality control conditions, this kit guarantees robust performance and ease of use, making it a reliable choice for both research and clinical studies. Count on Assay Genie's BMP-1 ELISA Kit for precise and consistent quantitation of this important protein in your research endeavors.
Product Name:
Human BMP-1 (Bone Morphogenetic Protein 1) ELISA Kit
SKU:
AEES00080
Target:
Human BMP-1 (Bone Morphogenetic Protein 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human BMP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BMP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BMP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human BMP-1. You can calculate the concentration of Human BMP-1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-113
92-105
100-112
Average (%)
105
97
104
1:4
Range (%)
83-97
95-108
98-109
Average (%)
90
100
104
1:8
Range (%)
93-104
84-99
89-102
Average (%)
99
92
95
1:16
Range (%)
94-107
92-104
90-98
Average (%)
100
96
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-96
92
EDTA plasma (n=5)
96-106
102
Cell culture media (n=5)
83-95
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
41.34
106.75
420.88
47.9
102.62
402.75
Standard deviation
2.16
4.85
21.89
2.56
5.13
16.11
C V (%)
5.22
4.54
5.2
5.34
5.0
4.0
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human BMP-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human BMP-1 in samples. No significant cross-reactivity or interference between Human BMP-1 and analogues was observed.
