Human BMP-15 (Bone Morphogenetic Protein 15) CLIA Kit
The Human BMP-15 (Bone Morphogenetic Protein-15) CLIA Kit is a powerful tool for the quantitative measurement of BMP-15 levels in human samples such as serum and plasma. This kit offers exceptional sensitivity and specificity, enabling researchers to obtain accurate and reproducible results for their studies.BMP-15 is a member of the bone morphogenetic protein family that plays a critical role in folliculogenesis and oocyte maturation. Dysregulation of BMP-15 has been linked to various reproductive disorders, making it a valuable biomarker for studying female fertility and potential treatments.
With its precision and reliability, the Human BMP-15 CLIA Kit is an indispensable resource for researchers investigating the role of BMP-15 in reproductive health and exploring its therapeutic potential. Unlock new insights into female fertility with this cutting-edge assay kit from Assay Genie.
Product Name:
Human BMP-15 (Bone Morphogenetic Protein 15) CLIA Kit
SKU:
HUES00395
Target:
Human BMP-15 (Bone Morphogenetic Protein 15)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human BMP-15. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BMP-15 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BMP-15, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human BMP-15. You can calculate the concentration of Human BMP-15 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-99
90-103
97-111
Average (%)
90
95
103
1:4
Range (%)
85-101
103-120
88-103
Average (%)
92
109
94
1:8
Range (%)
99-111
84-96
99-111
Average (%)
105
91
105
1:16
Range (%)
93-105
97-111
91-105
Average (%)
98
104
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
97
EDTA plasma (n=5)
84-95
90
Cell culture media (n=5)
97-109
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
93.07
182.83
789.88
100.01
184.42
743.52
Standard deviation
10.64
19.65
58.77
8.5
21.8
50.56
C V (%)
11.43
10.75
7.44
8.5
11.82
6.8
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human BMP-15 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human BMP-15 in samples. No significant cross-reactivity or interference between Human BMP-15 and analogues was observed.
