Human BMP-2 (Bone Morphogenetic Protein 2) CLIA Kit
The Human BMP-2 Bone Morphogenetic Protein 2 CLIA Kit is specifically designed to accurately measure levels of BMP-2 in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and consistent results for various research purposes.BMP-2, a member of the bone morphogenetic protein family, plays a key role in bone and cartilage formation, as well as in embryonic development. Dysregulation of BMP-2 has been linked to various diseases and conditions, including skeletal disorders, cancer, and cardiovascular diseases.
Therefore, accurate measurement of BMP-2 levels can provide valuable insights into these conditions and aid in the development of potential therapeutic interventions.With its reliable performance and ease of use, the Human BMP-2 Bone Morphogenetic Protein 2 CLIA Kit is an invaluable tool for researchers studying BMP-2 biology and its implications in human health and disease.
Product Name:
Human BMP-2 (Bone Morphogenetic Protein 2) CLIA Kit
SKU:
HUES00011
Target:
Human BMP-2 (Bone Morphogenetic Protein 2)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human BMP-2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BMP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human BMP-2. You can calculate the concentration of Human BMP-2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-98
100-114
90-102
Average (%)
91
105
96
1:4
Range (%)
85-97
91-103
103-116
Average (%)
92
97
110
1:8
Range (%)
94-110
102-116
98-114
Average (%)
100
108
106
1:16
Range (%)
86-100
91-106
101-118
Average (%)
92
97
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
101
EDTA plasma (n=5)
85-99
91
Cell culture media (n=5)
93-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
106.97
212.74
714.67
97.44
230.89
713.92
Standard deviation
10.29
16.15
48.38
11.99
18.52
60.83
C V (%)
9.62
7.59
6.77
12.31
8.02
8.52
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human BMP-2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human BMP-2 in samples. No significant cross-reactivity or interference between Human BMP-2 and analogues was observed.
