Human BMP-3 (Bone Morphogenetic Protein 3) CLIA Kit
The Human BMP-3 (Bone Morphogenetic Protein 3) CLIA Kit is a cutting-edge tool designed for precise measurement of BMP-3 levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers dependable and consistent results, making it an invaluable asset for various research applications.BMP-3 is a pivotal protein involved in bone development and regeneration, playing a crucial role in osteoblast differentiation and bone formation.
Dysregulation of BMP-3 has been linked to skeletal disorders and diseases such as osteoporosis, making it a vital biomarker for studying these conditions and exploring potential therapeutic interventions.Utilize the Human BMP-3 CLIA Kit to advance your research in bone biology, orthopedics, and regenerative medicine. With its reliability and precision, this kit is the perfect tool for unraveling the intricacies of BMP-3 biology and its implications in human health and disease.
Product Name:
Human BMP-3 (Bone Morphogenetic Protein 3) CLIA Kit
SKU:
HUES00210
Target:
Human BMP-3 (Bone Morphogenetic Protein 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human BMP-3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BMP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BMP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human BMP-3. You can calculate the concentration of Human BMP-3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-113
91-101
87-98
Average (%)
106
96
93
1:4
Range (%)
102-116
88-102
102-119
Average (%)
108
93
108
1:8
Range (%)
86-101
86-98
88-99
Average (%)
92
91
94
1:16
Range (%)
92-107
89-104
92-106
Average (%)
98
97
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
95
EDTA plasma (n=5)
89-105
97
Cell culture media (n=5)
84-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
107.7
188.51
834.78
107.36
203.81
761.17
Standard deviation
9.7
13.69
61.69
10.37
20.81
67.14
C V (%)
9.01
7.26
7.39
9.66
10.21
8.82
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human BMP-3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human BMP-3 in samples. No significant cross-reactivity or interference between Human BMP-3 and analogues was observed.
