Human BMP-4 (Bone Morphogenetic Protein 4) CLIA Kit
The Human BMP-4 (Bone Morphogenetic Protein-4) CLIA Kit is a cutting-edge diagnostic tool designed for the precise measurement of BMP-4 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.BMP-4 is a key regulator in the bone morphogenetic protein family, playing a vital role in skeletal development, cell differentiation, and tissue repair. Dysregulation of BMP-4 has been implicated in various diseases such as osteoporosis, cancer, and developmental disorders, underscoring its importance as a biomarker for disease research and therapeutic development.
With the Human BMP-4 CLIA Kit, researchers can confidently investigate the role of BMP-4 in health and disease, paving the way for innovative treatments and interventions targeting this crucial signaling molecule. Elevate your research with the precision and reliability of the Human BMP-4 CLIA Kit from AssayGenie.
Product Name:
Human BMP-4 (Bone Morphogenetic Protein 4) CLIA Kit
SKU:
HUES00012
Target:
Human BMP-4 (Bone Morphogenetic Protein 4)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human BMP-4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BMP-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BMP-4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human BMP-4. You can calculate the concentration of Human BMP-4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-118
85-97
93-108
Average (%)
107
91
99
1:4
Range (%)
88-101
87-99
100-116
Average (%)
95
93
106
1:8
Range (%)
93-109
103-118
88-103
Average (%)
100
110
94
1:16
Range (%)
86-101
85-100
90-106
Average (%)
92
91
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
95
EDTA plasma (n=5)
96-114
104
Cell culture media (n=5)
91-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
55.04
148.59
372.42
55.23
146.01
388.64
Standard deviation
6.02
15.29
23.87
5.04
14.86
44.42
C V (%)
10.94
10.29
6.41
9.13
10.18
11.43
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human BMP-4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human BMP-4 in samples. No significant cross-reactivity or interference between Human BMP-4 and analogues was observed.
