Human BMP-6 (Bone Morphogenetic Protein 6) ELISA Kit
The Human BMP-6 (Bone Morphogenetic Protein 6) ELISA Kit is specifically designed to quantitatively measure BMP-6 levels in various human biological samples. BMP-6, a member of the transforming growth factor beta (TGF-β) superfamily, is a crucial signaling molecule with diverse functions in cell growth, differentiation, and embryonic development.
It plays an essential role in bone formation, cellular proliferation, and differentiation, making it a key regulator of skeletal development and maintenance. Accurate measurement of BMP-6 is vital for investigating its involvement in bone biology, cell signaling, and tissue regeneration. This ELISA kit offers exceptional sensitivity and specificity, ensuring precise and reliable results. Manufactured under stringent quality control standards, the kit delivers robust performance and user-friendly protocols, making it suitable for both research and clinical applications. Rely on Assay Genie's BMP-6 ELISA Kit for accurate and consistent quantification of this significant protein in your research endeavors.
Product Name:
Human BMP-6 (Bone Morphogenetic Protein 6) ELISA Kit
SKU:
AEES00082
Target:
Human BMP-6 (Bone Morphogenetic Protein 6)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human BMP-6. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BMP-6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BMP-6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human BMP-6. You can calculate the concentration of Human BMP-6 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-105
92-103
88-98
Average (%)
99
100
93
1:4
Range (%)
85-95
90-101
92-103
Average (%)
92
94
100
1:8
Range (%)
101-109
97-106
91-102
Average (%)
104
100
99
1:16
Range (%)
93-108
98-111
87-101
Average (%)
101
105
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
100
EDTA plasma (n=5)
95-111
103
Cell culture media (n=5)
92-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
40.09
122.13
458.34
41.53
105.65
456.97
Standard deviation
2.14
5.63
24.11
2.17
4.84
24.13
C V (%)
5.34
4.61
5.26
5.23
4.58
5.28
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human BMP-6 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human BMP-6 in samples. No significant cross-reactivity or interference between Human BMP-6 and analogues was observed.
