Human BMP-7 (Bone Morphogenetic Protein 7) CLIA Kit
The Human BMP-7 (Bone Morphogenetic Protein 7) CLIA Kit is specifically designed for the accurate measurement of BMP-7 levels in human samples such as serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring precise and consistent results for a variety of research purposes.BMP-7 is a key protein in the regulation of bone and cartilage development, as well as in the process of tissue regeneration and repair. It plays a crucial role in conditions such as bone disorders, osteoporosis, and tissue injuries, making it a valuable biomarker for investigating these conditions and developing potential therapeutic interventions.
With the Human BMP-7 CLIA Kit, researchers can confidently study the role of BMP-7 in various physiological and pathophysiological processes, paving the way for new insights and advancements in the field of bone biology and regenerative medicine.
Product Name:
Human BMP-7 (Bone Morphogenetic Protein 7) CLIA Kit
SKU:
HUES00013
Target:
Human BMP-7 (Bone Morphogenetic Protein 7)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human BMP-7. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BMP-7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BMP-7, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human BMP-7. You can calculate the concentration of Human BMP-7 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-99
86-100
94-109
Average (%)
94
91
100
1:4
Range (%)
94-110
86-101
102-118
Average (%)
101
92
110
1:8
Range (%)
102-119
97-111
94-108
Average (%)
109
104
101
1:16
Range (%)
93-104
89-101
89-101
Average (%)
98
95
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-113
103
EDTA plasma (n=5)
92-104
97
Cell culture media (n=5)
99-112
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
55.17
142.81
347.13
51.82
146.11
346.24
Standard deviation
6.63
16.97
28.22
6.24
14.86
25.1
C V (%)
12.02
11.88
8.13
12.04
10.17
7.25
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human BMP-7 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human BMP-7 in samples. No significant cross-reactivity or interference between Human BMP-7 and analogues was observed.
