Human BPI (Bactericidal/Permeability Increasing Protein) CLIA Kit
The Human BPI (Bactericidal Permeability-Increasing Protein) CLIA Kit is specifically designed for the precise measurement of BPI levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.BPI is a vital protein involved in innate immunity, playing a key role in combating bacterial infections and enhancing immune response.
It is crucial in various inflammatory conditions and immune-related diseases, making it an important biomarker for studying these conditions and potential therapeutic interventions.Overall, the Human BPI CLIA Kit provides researchers with a reliable tool for investigating BPI levels in different biological samples, furthering our understanding of immune responses and inflammatory processes.
Product Name:
Human BPI (Bactericidal/Permeability Increasing Protein) CLIA Kit
SKU:
HUES00379
Target:
Human BPI (Bactericidal/Permeability Increasing Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human BPI. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BPI and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BPI, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human BPI. You can calculate the concentration of Human BPI in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
92-105
88-100
Average (%)
98
98
95
1:4
Range (%)
90-103
91-107
93-105
Average (%)
96
98
99
1:8
Range (%)
85-97
88-102
103-117
Average (%)
90
94
109
1:16
Range (%)
88-100
96-109
99-111
Average (%)
93
102
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
98
EDTA plasma (n=5)
91-102
97
Cell culture media (n=5)
93-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
38.22
95.59
360.56
34.45
90.49
343.69
Standard deviation
4.35
6.74
22.21
4.47
10.41
38.6
C V (%)
11.38
7.05
6.16
12.98
11.5
11.23
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human BPI concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human BPI in samples. No significant cross-reactivity or interference between Human BPI and analogues was observed.
