Human Brain Abundant, Membrane Attached Signal Protein 1 (BASP1) ELISA Kit
The Human Brain Abundant Membrane-Attached Signal Protein 1 (BASP1) ELISA Kit is a valuable tool for researchers looking to accurately measure levels of BASP1 in human samples such as serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.BASP1 is a membrane-bound protein that plays a crucial role in cellular signaling and gene regulation within the brain. It is involved in processes such as neuronal differentiation, neuronal plasticity, and axon growth, making it a key player in neurological development and function.
Dysregulation of BASP1 has been implicated in various neurological disorders, making it an important biomarker for studying these conditions and potential therapeutic interventions.With its superior performance and ease of use, the Human Brain Abundant Membrane-Attached Signal Protein 1 (BASP1) ELISA Kit is an essential tool for researchers striving to advance our understanding of brain function and neurological disorders.
Product Name:
Human Brain Abundant, Membrane Attached Signal Protein 1 (BASP1) ELISA Kit
Product Code:
HUDL00311
Size:
96 Assays
Target:
BASP1
Synonyms:
CAP-23, NAP-22, Brain acid soluble protein 1, 22 kDa neuronal tissue-enriched acidic protein, Neuronal axonal membrane protein NAP-22
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
Wiskott et al.
Blood proteome of acute intracranial hemorrhage in infant victims of abusive head trauma
