Human Breast Cancer Susceptibility Protein 1 (BRCA1) ELISA Kit
The Human BRCA1 (Breast Cancer Susceptibility Protein 1) ELISA Kit is specifically designed for the accurate detection of BRCA1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.BRCA1 is a critical protein involved in DNA repair and maintenance, particularly in the context of breast and ovarian cancer susceptibility. Mutations in the BRCA1 gene are associated with an increased risk of developing these types of cancers, making BRCA1 a key biomarker for studying cancer pathogenesis and potential therapeutic interventions.
By utilizing the Human BRCA1 ELISA Kit, researchers can conveniently measure BRCA1 levels in biological samples, gaining valuable insights into the role of this protein in cancer development and progression. This kit provides a valuable tool for advancing research in the field of oncology and personalized medicine.
Product Name:
Human Breast Cancer Susceptibility Protein 1 (BRCA1) ELISA Kit
Product Code:
HUDL00354
Size:
96 Assays
Target:
BRCA1
Synonyms:
BRCAI, BRCC1, IRIS, PSCP, RNF53, Breast Cancer 1,Early Onset, RING finger protein 53
Detection Method:
Sandwich
Range:
31.25-2000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
