The Human BSP (Bone Sialoprotein) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of BSP levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for various research applications in the field of bone biology.BSP is a key protein found in bone and other tissues that plays a crucial role in bone formation and mineralization.
Abnormal levels of BSP have been associated with various bone diseases and disorders, making it a valuable biomarker for studying conditions such as osteoporosis, osteoarthritis, and bone metastasis in cancer.By utilizing the Human BSP CLIA Kit, researchers can gain insights into the role of BSP in bone health and disease, paving the way for potential therapeutic interventions and improved patient outcomes.
Product Name:
Human BSP (Bone Sialoprotein) CLIA Kit
SKU:
HUES00397
Target:
Human BSP (Bone Sialoprotein)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human BSP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BSP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BSP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human BSP. You can calculate the concentration of Human BSP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-112
98-114
92-103
Average (%)
105
105
98
1:4
Range (%)
87-101
102-119
87-101
Average (%)
94
109
92
1:8
Range (%)
85-99
96-110
97-111
Average (%)
90
103
103
1:16
Range (%)
93-108
87-99
97-112
Average (%)
99
92
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
94
EDTA plasma (n=5)
87-101
93
Cell culture media (n=5)
84-100
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
421.64
747.01
2955.41
442.03
784.2
2774.14
Standard deviation
52.54
77.46
279.29
53.49
71.13
170.61
C V (%)
12.46
10.37
9.45
12.1
9.07
6.15
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human BSP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human BSP in samples. No significant cross-reactivity or interference between Human BSP and analogues was observed.
