The Human BTC (Betacellulin) CLIA Kit is a highly sensitive and specific assay designed for the precise quantification of Betacellulin levels in human biological samples. This kit is ideal for researchers studying the role of Betacellulin in various physiological processes, including cell proliferation and differentiation.Betacellulin is a key growth factor that plays a crucial role in cell growth and development, particularly in the pancreas and other epithelial tissues. Dysregulation of Betacellulin has been associated with various diseases, making it a valuable biomarker for research and potential therapeutic interventions.
The Human BTC (Betacellulin) CLIA Kit from Assay Genie offers reliable and reproducible results, making it a valuable tool for investigating the role of Betacellulin in health and disease. With its high sensitivity and specificity, this kit is essential for researchers looking to advance their understanding of Betacellulin's functions and implications in various pathologies.
Product Name:
Human bTC (Betacellulin) CLIA Kit
SKU:
HUES00386
Target:
Human bTC (Betacellulin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human bTC. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human bTC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human bTC, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human bTC. You can calculate the concentration of Human bTC in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
88-103
85-100
Average (%)
99
96
91
1:4
Range (%)
96-106
88-101
94-105
Average (%)
101
96
99
1:8
Range (%)
89-104
95-108
93-104
Average (%)
96
100
98
1:16
Range (%)
96-114
95-110
86-97
Average (%)
104
102
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
99
EDTA plasma (n=5)
96-110
102
Cell culture media (n=5)
100-116
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
41.09
85.91
326.16
38.39
77.94
333.87
Standard deviation
3.78
8.55
26.88
4.81
6.98
21.0
C V (%)
9.2
9.95
8.24
12.53
8.96
6.29
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human bTC concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human bTC in samples. No significant cross-reactivity or interference between Human bTC and analogues was observed.
