The Human C-P Peptide CLIA Kit is a cutting-edge assay designed for the precise measurement of C-P peptide levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit guarantees accurate and reliable results, making it an invaluable tool for various research applications.C-P peptide is a key biomarker involved in the regulation of insulin secretion and glucose metabolism, making it essential for studying diabetes and other metabolic disorders.
By accurately measuring C-P peptide levels, researchers can gain valuable insights into the pathophysiology of these conditions and explore potential therapeutic interventions.Overall, the Human C-P Peptide CLIA Kit offers researchers a powerful tool to advance their understanding of metabolic disorders and develop targeted treatments, leading to improved outcomes for patients.
Product Name:
Human C-P (C-Peptide) CLIA Kit
SKU:
HUES00114
Target:
Human C-P (C-Peptide)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human C-P. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C-P and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C-P, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human C-P. You can calculate the concentration of Human C-P in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-99
102-117
87-102
Average (%)
92
108
93
1:4
Range (%)
92-103
93-109
100-116
Average (%)
97
101
107
1:8
Range (%)
84-99
102-117
86-100
Average (%)
90
109
92
1:16
Range (%)
94-107
87-99
99-113
Average (%)
99
94
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
99
EDTA plasma (n=5)
90-103
96
Cell culture media (n=5)
97-110
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
210.85
337.83
1593.39
212.97
338.05
1751.11
Standard deviation
26.44
34.63
160.14
17.55
40.46
163.03
C V (%)
12.54
10.25
10.05
8.24
11.97
9.31
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human C-P concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human C-P in samples. No significant cross-reactivity or interference between Human C-P and analogues was observed.
