The Human C1INH (Complement 1 Inhibitor) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of C1INH levels in human samples. This kit is ideal for researchers studying the complement system and its role in immune response and inflammation.C1INH is a key regulator of the complement system, inhibiting the activation of complement proteins and preventing excessive inflammation. Dysregulation of C1INH has been associated with autoimmune diseases, inflammatory conditions, and hereditary angioedema, making it a valuable biomarker for understanding and treating these disorders.
With its reliable and reproducible results, the Human C1INH CLIA Kit is a valuable tool for research in immunology, inflammation, and related fields. Get accurate and precise measurements of C1INH levels with this easy-to-use kit from Assay Genie.
Product Name:
Human C1INH (Complement 1 Inhibitor) CLIA Kit
SKU:
HUES00499
Target:
Human C1INH (Complement 1 Inhibitor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human C1INH. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C1INH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C1INH, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human C1INH. You can calculate the concentration of Human C1INH in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
90-101
100-112
Average (%)
97
95
107
1:4
Range (%)
94-106
91-104
90-104
Average (%)
100
97
97
1:8
Range (%)
100-116
97-110
98-114
Average (%)
109
102
105
1:16
Range (%)
97-111
99-110
97-110
Average (%)
103
104
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-97
92
EDTA plasma (n=5)
90-102
96
Cell culture media (n=5)
86-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.55
1.17
4.78
0.57
1.21
4.47
Standard deviation
0.05
0.12
0.51
0.06
0.1
0.27
C V (%)
9.09
10.26
10.67
10.53
8.26
6.04
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human C1INH concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human C1INH in samples. No significant cross-reactivity or interference between Human C1INH and analogues was observed.
