The Human C1q (Complement 1q) ELISA Kit is a specialized assay designed for the accurate quantification of Complement 1q levels in biological samples. C1q is a fundamental component of the complement system, playing a critical role in immune response regulation and host defense. By participating in immune complex clearance and recognizing pathogens, C1q serves as a key mediator of innate immunity. This ELISA kit enables the quantitative measurement of C1q levels, providing valuable insights into immune responses, inflammatory processes, and immune-related disorders.
Accurate measurement of C1q is crucial for understanding its involvement in immune surveillance, autoimmune conditions, and infectious diseases. The C1q ELISA Kit offered by Assay Genie ensures exceptional sensitivity and specificity, delivering reliable and reproducible results. With robust performance and user-friendly protocols, this kit is suitable for various research and clinical applications. Rely on Assay Genie's C1q ELISA Kit for precise and consistent quantification of this essential immune biomarker in your investigations.
Product Name:
Human C1q (Complement 1q) ELISA Kit
SKU:
AEES00089
Target:
Human C1q (Complement 1q)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human C1q. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C1q and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C1q, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human C1q. You can calculate the concentration of Human C1q in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-105
101-109
86-98
Average (%)
97
105
91
1:4
Range (%)
99-109
88-100
94-104
Average (%)
103
95
100
1:8
Range (%)
87-99
90-99
92-102
Average (%)
92
95
98
1:16
Range (%)
92-104
86-98
82-93
Average (%)
99
94
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
83-98
91
EDTA plasma (n=5)
85-100
92
Cell culture media (n=5)
93-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.88
11.02
45.1
4.4
11.3
45.98
Standard deviation
0.28
0.52
2.08
0.25
0.52
2.21
C V (%)
5.74
4.72
4.61
5.68
4.6
4.81
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human C1q concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human C1q in samples. No significant cross-reactivity or interference between Human C1q and analogues was observed.
