The Human C2 (Complement Component 2) ELISA Kit is meticulously engineered for the precise quantification of C2 in various biological samples. C2 is a pivotal factor in the classical complement pathway, contributing to host defense, immune surveillance, and inflammation modulation.
As an essential component of the complement system, C2 plays a critical role in immune complex processing, opsonization, and the formation of the membrane-attack complex. Understanding C2 levels is crucial for unraveling its implications in infection, autoimmunity, and inflammatory responses. Accurate measurement of C2 is essential in elucidating its function in immunity and disease mechanisms. With outstanding sensitivity and specificity, Assay Genie's C2 ELISA Kit ensures reliable and reproducible results. Manufactured under stringent quality control standards, this kit offers robust performance and ease of use, making it an ideal choice for both research and clinical applications. Researchers can trust Assay Genie's C2 ELISA Kit for precise quantification of this crucial complement component in their studies.
Product Name:
Human C2 (Complement Component 2) ELISA Kit
SKU:
AEES00091
Target:
Human C2 (Complement Component 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human C2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human C2. You can calculate the concentration of Human C2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-105
94-109
86-99
Average (%)
97
102
92
1:4
Range (%)
101-107
93-106
99-109
Average (%)
104
98
102
1:8
Range (%)
89-100
95-101
91-103
Average (%)
92
98
96
1:16
Range (%)
83-99
100-106
100-111
Average (%)
91
103
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-112
104
EDTA plasma (n=5)
91-100
97
Cell culture media (n=5)
89-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.29
11.09
45.63
4.37
10.1
48.62
Standard deviation
0.23
0.51
2.12
0.25
0.49
2.29
C V (%)
5.36
4.6
4.65
5.72
4.85
4.71
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human C2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human C2 in samples. No significant cross-reactivity or interference between Human C2 and analogues was observed.
