The Human C4a Complement Component 4a CLIA Kit is a reliable and efficient tool for measuring levels of C4a complement component 4a in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit offers accurate and reproducible results for a variety of research applications.C4a complement component 4a is a key player in the complement system, a crucial part of the immune response that helps to identify and eliminate pathogens and damaged cells. Dysregulation of the complement system, including abnormalities in C4a levels, has been implicated in various health conditions such as autoimmune diseases, inflammatory disorders, and infections.
Therefore, measuring C4a levels can provide valuable insights into the immune system's functioning and the development of related diseases.Whether you are studying immune responses in disease conditions, evaluating treatment responses, or exploring potential therapeutic targets, the Human C4a Complement Component 4a CLIA Kit is a valuable tool for advancing your research. Trust in its accuracy and precision to support your investigations and discoveries in the field of immunology and beyond.
Product Name:
Human C4a (Complement Component 4a) CLIA Kit
SKU:
HUES00163
Target:
Human C4a (Complement Component 4a)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human C4a. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C4a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C4a, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human C4a. You can calculate the concentration of Human C4a in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-117
96-109
87-98
Average (%)
107
103
93
1:4
Range (%)
103-118
91-103
86-100
Average (%)
109
98
91
1:8
Range (%)
86-98
86-97
90-108
Average (%)
92
91
98
1:16
Range (%)
100-118
100-111
88-103
Average (%)
108
105
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-104
97
EDTA plasma (n=5)
100-115
108
Cell culture media (n=5)
101-116
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.93
1.76
6.74
0.89
1.75
6.6
Standard deviation
0.1
0.14
0.45
0.1
0.2
0.61
C V (%)
10.75
7.95
6.68
11.24
11.43
9.24
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human C4a concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human C4a in samples. No significant cross-reactivity or interference between Human C4a and analogues was observed.
