Human C4BP beta (C4 Binding Protein Beta) CLIA Kit
The Human C4BP Beta (C4 Binding Protein Beta) CLIA Kit is designed for the precise measurement of C4BP Beta levels in human samples. This kit provides a high level of accuracy and reliability, allowing for consistent and reproducible results across various research applications. C4BP Beta is a critical protein that plays a role in regulating the complement system, a crucial part of the immune response. Dysregulation of this system can lead to various diseases, including autoimmune disorders and inflammatory conditions.
Monitoring C4BP Beta levels can provide valuable insights into the immune response and aid in the development of targeted therapies. The Human C4BP Beta CLIA Kit from Assay Genie offers researchers a powerful tool for studying the role of C4BP Beta in health and disease, facilitating advancements in the field of immunology and autoimmune research.
Product Name:
Human C4BP beta (C4 Binding Protein Beta) CLIA Kit
SKU:
HUES00282
Target:
Human C4BP beta (C4 Binding Protein Beta)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
56.25 pg/mL
Detection range:
93.75-6000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human C4BPβ. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C4BPβ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C4BPβ, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human C4BPβ. You can calculate the concentration of Human C4BPβ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
96-110
93-103
Average (%)
99
104
98
1:4
Range (%)
102-116
100-115
93-108
Average (%)
109
107
100
1:8
Range (%)
96-109
100-112
88-100
Average (%)
103
106
95
1:16
Range (%)
97-113
89-103
100-115
Average (%)
103
96
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-101
92
EDTA plasma (n=5)
86-98
91
Cell culture media (n=5)
84-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
330.75
664.08
2502.92
313.63
635.11
2515.26
Standard deviation
39.59
55.05
164.94
26.88
50.24
193.68
C V (%)
11.97
8.29
6.59
8.57
7.91
7.7
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human C4BPβ concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human C4BPβ in samples. No significant cross-reactivity or interference between Human C4BPβ and analogues was observed.
