The Human CA2 (Carbonic Anhydrase II) CLIA Kit is a highly sensitive assay designed for the quantitative measurement of CA2 levels in human serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it an excellent tool for research applications in various fields.Carbonic Anhydrase II is an essential enzyme involved in regulating pH levels and carbon dioxide transport in the body. Dysregulation of CA2 has been implicated in a variety of diseases, including osteoporosis, glaucoma, and certain cancers.
By studying CA2 levels, researchers can gain valuable insights into these conditions and potentially identify new therapeutic targets.With its high sensitivity and specificity, the Human CA2 CLIA Kit offers a reliable solution for detecting and quantifying Carbonic Anhydrase II levels, making it a valuable asset for research laboratories and clinical settings alike.
Product Name:
Human CA2 (Carbonic Anhydrase II) CLIA Kit
SKU:
HUES00424
Target:
Human CA2 (Carbonic Anhydrase II)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CA2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CA2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CA2. You can calculate the concentration of Human CA2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
89-101
97-113
Average (%)
103
96
103
1:4
Range (%)
88-100
86-97
103-118
Average (%)
95
92
110
1:8
Range (%)
96-110
88-101
90-107
Average (%)
104
93
97
1:16
Range (%)
91-108
87-102
99-115
Average (%)
99
94
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-106
101
EDTA plasma (n=5)
95-109
102
Cell culture media (n=5)
90-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.0
5.04
15.04
2.14
5.4
16.47
Standard deviation
0.17
0.47
1.51
0.25
0.57
1.15
C V (%)
8.5
9.33
10.04
11.68
10.56
6.98
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CA2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CA2 in samples. No significant cross-reactivity or interference between Human CA2 and analogues was observed.
