The Human CA724 Tumor Marker CA724 CLIA Kit is specifically designed for the precise measurement of CA724 levels in human serum and plasma samples. This kit offers exceptional sensitivity and accuracy, guaranteeing dependable and consistent results for various research purposes. CA724, a tumor marker, is associated with various types of cancer, particularly gastrointestinal and breast cancers. Monitoring CA724 levels can aid in early detection, prognosis assessment, and treatment monitoring for these malignancies, highlighting its importance in clinical research and patient care.
With its advanced technology and superior performance, the Human CA724 Tumor Marker CA724 CLIA Kit is a valuable tool for researchers and healthcare professionals seeking to enhance their understanding of cancer biology and improve patient outcomes.
Product Name:
Human CA724 (Tumor Marker CA724) CLIA Kit
SKU:
HUES00407
Target:
Human CA724 (Tumor Marker CA724)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CA724. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CA724 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CA724, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CA724. You can calculate the concentration of Human CA724 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-106
85-98
95-110
Average (%)
98
92
101
1:4
Range (%)
102-117
86-98
94-105
Average (%)
107
92
100
1:8
Range (%)
97-109
99-113
88-101
Average (%)
103
106
94
1:16
Range (%)
93-109
102-115
96-110
Average (%)
99
108
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-115
106
EDTA plasma (n=5)
90-105
97
Cell culture media (n=5)
96-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
183.97
538.47
1665.88
183.24
505.86
1697.57
Standard deviation
21.12
53.04
138.6
21.46
48.92
127.15
C V (%)
11.48
9.85
8.32
11.71
9.67
7.49
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CA724 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CA724 in samples. No significant cross-reactivity or interference between Human CA724 and analogues was observed.
