The Human CA724 Tumor Marker ELISA Kit is specifically designed for the precise detection of CA724 levels in human serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, guaranteeing accurate and consistent results, making it an excellent choice for various research purposes.CA724 is a tumor marker associated with various cancers, including breast, lung, ovarian, and gastrointestinal cancers. Monitoring CA724 levels can aid in early detection, prognosis, and treatment response evaluation in cancer patients, making it a valuable tool for cancer research and clinical practice.
This ELISA kit from Assay Genie offers researchers a reliable and efficient method for quantifying CA724 levels in biological samples, enabling further investigation into the role of this tumor marker in cancer development and progression. Upgrade your research capabilities with the Human CA724 Tumor Marker ELISA Kit today.
Product Name:
Human CA724 (Tumor Marker CA724) ELISA Kit
SKU:
HUES01779
Target:
Human CA724 (Tumor Marker CA724)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CA724. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CA724 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CA724, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CA724. You can calculate the concentration of Human CA724 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-112
91-102
97-110
Average (%)
102
97
102
1:4
Range (%)
87-100
83-96
86-98
Average (%)
92
88
93
1:8
Range (%)
92-105
84-95
82-94
Average (%)
99
89
89
1:16
Range (%)
92-103
84-99
84-94
Average (%)
97
90
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-103
94
EDTA plasma (n=5)
94-106
99
Cell culture media (n=5)
85-98
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.1
3.17
9.03
1.14
2.94
8.77
Standard deviation
0.07
0.16
0.35
0.07
0.16
0.3
C V (%)
6.36
5.05
3.88
6.14
5.44
3.42
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CA724 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CA724 in samples. No significant cross-reactivity or interference between Human CA724 and analogues was observed.
