The Human CA9 (Carbonic Anhydrase IX) CLIA Kit is specifically designed for the quantitative measurement of CA9 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and accuracy, providing researchers with reliable and reproducible results for a variety of research purposes.Carbonic Anhydrase IX (CA9) is an important enzyme involved in pH regulation and tumor progression. Its overexpression has been linked to various cancer types, making it a valuable biomarker for cancer research and potential therapeutic targets.
By accurately measuring CA9 levels, researchers can gain valuable insights into cancer development and progression, facilitating the development of targeted treatments and personalized medicine approaches.Overall, the Human CA9 (Carbonic Anhydrase IX) CLIA Kit is a valuable tool for researchers investigating cancer biology, tumor microenvironment, and therapeutic strategies. Its high sensitivity and specificity make it an ideal choice for accurate and reliable CA9 detection, enabling advancements in cancer research and drug development.
Product Name:
Human CA9 (Carbonic Anhydrase IX) CLIA Kit
SKU:
HUES00015
Target:
Human CA9 (Carbonic Anhydrase IX)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CA9. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CA9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CA9, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CA9. You can calculate the concentration of Human CA9 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-116
95-111
85-99
Average (%)
107
102
91
1:4
Range (%)
94-111
103-116
96-109
Average (%)
101
109
104
1:8
Range (%)
87-100
101-116
88-100
Average (%)
92
109
95
1:16
Range (%)
94-109
92-104
98-116
Average (%)
101
97
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-99
92
EDTA plasma (n=5)
89-104
97
Cell culture media (n=5)
94-107
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
39.2
76.13
270.93
35.47
81.46
249.33
Standard deviation
4.03
5.7
25.12
3.55
9.18
21.92
C V (%)
10.28
7.49
9.27
10.01
11.27
8.79
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CA9 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CA9 in samples. No significant cross-reactivity or interference between Human CA9 and analogues was observed.
