Human cADPRH/CD38 (Cyclic ADP Ribose Hydrolase) CLIA Kit
The Human CD38 (Cyclic ADP-Ribose Hydrolase) CLIA Kit is specifically designed for the precise measurement of CD38 levels in human samples, including serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.CD38 is an important enzyme involved in the metabolism of cyclic ADP-ribose, a molecule that plays a role in intracellular calcium homeostasis and signaling.
Dysregulation of CD38 has been linked to various diseases, including cancer, autoimmune disorders, and neurodegenerative conditions. As such, measuring CD38 levels can provide valuable insights into disease pathogenesis and potential therapeutic interventions.With this advanced CLIA kit, researchers can gain a deeper understanding of CD38 function and its implications in disease progression. Trust in the accuracy and reliability of the Human CD38 CLIA Kit for your research needs.
Product Name:
Human cADPRH/CD38 (Cyclic ADP Ribose Hydrolase) CLIA Kit
SKU:
HUES00923
Target:
Human cADPRH/CD38 (Cyclic ADP Ribose Hydrolase)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human cADPRH/CD38. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human cADPRH/CD38 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human cADPRH/CD38, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human cADPRH/CD38. You can calculate the concentration of Human cADPRH/CD38 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-114
104-119
94-105
Average (%)
104
110
100
1:4
Range (%)
86-100
93-105
89-101
Average (%)
93
100
95
1:8
Range (%)
97-110
87-98
89-102
Average (%)
103
92
96
1:16
Range (%)
94-108
93-108
93-109
Average (%)
99
99
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-112
103
EDTA plasma (n=5)
97-113
104
Cell culture media (n=5)
99-112
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.08
2.73
8.03
1.07
2.76
8.15
Standard deviation
0.12
0.27
0.81
0.1
0.31
0.57
C V (%)
11.11
9.89
10.09
9.35
11.23
6.99
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human cADPRH/CD38 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human cADPRH/CD38 in samples. No significant cross-reactivity or interference between Human cADPRH/CD38 and analogues was observed.
