The Human CALD (Caldesmon) CLIA Kit is specifically designed for the quantitative detection of caldesmon levels in human samples such as serum and plasma. This kit offers high sensitivity and accuracy, allowing for reliable and reproducible results for research purposes.Caldesmon is a key regulatory protein in smooth muscle cells and plays a vital role in controlling muscle contraction. Dysregulation of caldesmon has been implicated in various diseases such as cancer and cardiovascular disorders, making it a valuable biomarker for studying these conditions and developing targeted therapies.
With the Human CALD CLIA Kit, researchers can confidently analyze caldesmon levels in human samples, gaining insights into its role in disease pathogenesis and potential therapeutic targets. This kit is a valuable tool for advancing research in the fields of oncology, cardiovascular health, and beyond.
Product Name:
Human CALD (Caldesmon) CLIA Kit
SKU:
HUES00411
Target:
Human CALD (Caldesmon)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CALD. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CALD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CALD, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CALD. You can calculate the concentration of Human CALD in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-102
86-98
102-118
Average (%)
96
91
110
1:4
Range (%)
87-99
89-103
99-115
Average (%)
92
95
105
1:8
Range (%)
102-117
90-105
89-100
Average (%)
109
96
94
1:16
Range (%)
90-102
87-99
93-107
Average (%)
96
93
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-108
101
EDTA plasma (n=5)
87-99
92
Cell culture media (n=5)
87-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
205.71
395.75
1606.28
194.65
382.27
1541.84
Standard deviation
17.3
36.92
143.44
20.57
30.08
100.84
C V (%)
8.41
9.33
8.93
10.57
7.87
6.54
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CALD concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CALD in samples. No significant cross-reactivity or interference between Human CALD and analogues was observed.
