The Human Calu-Calumenin CLIA Kit is a cutting-edge assay designed for the precise measurement of calu-calumenin levels in human biological samples such as serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers accurate and reproducible results, making it an indispensable tool for various research applications.Calu-calumenin is a vital protein involved in calcium binding and plays a crucial role in various cellular processes such as cell signaling, protein folding, and gene transcription regulation. Dysregulation of calu-calumenin has been linked to several diseases, including cancer, Alzheimer's, and diabetes, making it a valuable biomarker for understanding these conditions and exploring potential therapeutic interventions.
The Human Calu-Calumenin CLIA Kit provides researchers with a reliable and efficient method for quantifying calu-calumenin levels, enabling in-depth investigations into its function and potential clinical implications. With its advanced technology and user-friendly protocols, this kit is an essential tool for advancing scientific knowledge and medical research in the field of calu-calumenin biology.
Product Name:
Human CALU (Calumenin) CLIA Kit
SKU:
HUES00416
Target:
Human CALU (Calumenin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CALU. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CALU and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CALU, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CALU. You can calculate the concentration of Human CALU in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-113
97-111
86-99
Average (%)
106
104
91
1:4
Range (%)
88-101
93-107
92-106
Average (%)
95
99
97
1:8
Range (%)
96-110
93-104
85-97
Average (%)
104
99
92
1:16
Range (%)
104-118
84-99
95-109
Average (%)
110
90
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-104
96
EDTA plasma (n=5)
92-104
99
Cell culture media (n=5)
98-113
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
103.3
211.14
703.75
100.04
214.83
751.22
Standard deviation
8.58
20.73
62.21
8.62
24.3
48.3
C V (%)
8.31
9.82
8.84
8.62
11.31
6.43
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CALU concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CALU in samples. No significant cross-reactivity or interference between Human CALU and analogues was observed.
