Human Carbohydrate Antigen 72-4 (CA72-4) ELISA Kit
The Human Carbohydrate Antigen 72-4 (CA72-4) ELISA Kit is a reliable and accurate tool for detecting CA72-4 levels in human serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides consistent and reproducible results, making it the perfect choice for various research applications.CA72-4 is a carbohydrate antigen that is commonly used as a biomarker for various cancers, particularly gastrointestinal cancers. Its levels have been found to be elevated in patients with gastric and pancreatic cancer, making it a valuable marker for early detection and monitoring of these diseases.
By using the Human CA72-4 ELISA Kit, researchers can conveniently measure CA72-4 levels in biological samples, allowing for the study of cancer progression, treatment response, and disease monitoring. With its user-friendly protocols and robust performance, this ELISA kit is an essential tool for cancer research and clinical diagnostics.
Product Name:
Human Carbohydrate Antigen 72-4 (CA72-4) ELISA Kit
Product Code:
HUDL00403
Size:
96 Assays
Target:
CA72-4
Synonyms:
cancer Antigen 72-4
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
82-102
92
EDTA plasma (n=5)
90-101
95
Heparin plasma (n=5)
88-100
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
