Human Carcinoembryonic Antigen Related Cell Adhesion Molecule 7 (CEACAM7) ELISA Kit
The Human Carcinoembryonic Antigen-Related Cell Adhesion Molecule 7 (CEACAM7) ELISA Kit is a reliable tool for detecting levels of CEACAM7 in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for various research applications.CEACAM7 is a protein involved in cell adhesion and plays a role in inflammation, immunity, and tumor development.
Elevated levels of CEACAM7 have been associated with certain types of cancer, making it a valuable biomarker for studying cancer progression and developing targeted therapies.With the Human CEACAM7 ELISA Kit, researchers can easily measure CEACAM7 levels in human samples, contributing to a better understanding of its role in disease pathways and opening up possibilities for new diagnostic and therapeutic strategies.
Product Name:
Human Carcinoembryonic Antigen Related Cell Adhesion Molecule 7 (CEACAM7) ELISA Kit
Product Code:
HUDL00526
Size:
96 Assays
Target:
CEACAM7
Synonyms:
CGM2, CEA, Carcinoembryonic Antigen Gene Family Member 2
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
