Human Carnitine Palmitoyltransferase 2, Mitochondrial (CPT2) ELISA Kit
The Human Carnitine Palmitoyltransferase 2 (Mitochondrial) ELISA Kit is a reliable tool for measuring levels of CPT2 in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing accurate and reproducible results for a variety of research purposes.Carnitine palmitoyltransferase 2 (CPT2) is an essential enzyme involved in fatty acid metabolism within the mitochondria. Dysregulation of CPT2 activity has been linked to various metabolic disorders, including fatty acid oxidation disorders and muscle diseases.
This ELISA kit can help researchers better understand the role of CPT2 in these diseases and potentially identify new therapeutic targets.With its advanced technology and user-friendly design, the Human Carnitine Palmitoyltransferase 2 (Mitochondrial) ELISA Kit is an indispensable tool for studying CPT2 biology and exploring its potential implications in metabolic diseases.
Product Name:
Human Carnitine Palmitoyltransferase 2, Mitochondrial (CPT2) ELISA Kit
Product Code:
HUDL00663
Size:
96 Assays
Target:
CPT2
Synonyms:
CPTASE, Carnitine O-Palmitoyltransferase 2, Carnitine palmitoyltransferase II
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
