The Human CASP1 (Caspase-1) CLIA Kit is a highly sensitive and accurate assay designed for the detection of caspase-1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides researchers with reliable and reproducible results, making it suitable for a wide range of research applications.Caspase-1 is a key enzyme involved in inflammation and the immune response, playing a critical role in mediating the inflammatory process and initiating pyroptosis.
Dysregulation of caspase-1 activity has been linked to various inflammatory and autoimmune diseases, as well as infectious diseases.By accurately measuring caspase-1 levels, researchers can gain valuable insights into the underlying mechanisms of these diseases and potentially identify new therapeutic targets. The Human CASP1 (Caspase-1) CLIA Kit provides a valuable tool for studying caspase-1 biology and developing novel treatments for inflammatory and immune-related disorders.
Product Name:
Human CASP1 (Caspase 1) CLIA Kit
SKU:
HUES00016
Target:
Human CASP1 (Caspase 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
3.75 pg/mL
Detection range:
6.25-400 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CASP1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CASP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CASP1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CASP1. You can calculate the concentration of Human CASP1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-102
97-112
100-114
Average (%)
95
103
106
1:4
Range (%)
86-96
86-96
94-108
Average (%)
91
91
100
1:8
Range (%)
92-103
98-111
95-107
Average (%)
97
104
101
1:16
Range (%)
86-96
84-100
90-101
Average (%)
91
91
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-105
96
EDTA plasma (n=5)
90-104
97
Cell culture media (n=5)
93-106
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
18.81
49.91
189.93
18.27
45.54
182.67
Standard deviation
1.63
5.07
16.77
1.79
5.41
19.55
C V (%)
8.67
10.16
8.83
9.8
11.88
10.7
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CASP1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CASP1 in samples. No significant cross-reactivity or interference between Human CASP1 and analogues was observed.
