The Human CASP4 (Caspase-4) CLIA Kit is specifically designed for the quantitative detection of caspase-4 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it an excellent choice for various research applications.Caspase-4 is a key enzyme involved in the inflammatory response and cell death pathways. Its dysregulation has been linked to various diseases, including inflammatory disorders, autoimmune diseases, and cancer.
By measuring caspase-4 levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.Overall, the Human CASP4 CLIA Kit from Assay Genie provides researchers with a reliable tool for studying caspase-4 biology and its role in disease pathology. Its ease of use, accuracy, and precision make it a valuable asset in the laboratory setting.
Product Name:
Human CASP4 (Caspase 4) CLIA Kit
SKU:
HUES00436
Target:
Human CASP4 (Caspase 4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CASP4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CASP4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CASP4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CASP4. You can calculate the concentration of Human CASP4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
90-107
102-115
Average (%)
92
97
107
1:4
Range (%)
85-99
84-97
97-112
Average (%)
92
91
105
1:8
Range (%)
93-107
89-105
96-113
Average (%)
98
97
104
1:16
Range (%)
85-97
88-100
102-118
Average (%)
92
94
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-96
90
EDTA plasma (n=5)
94-107
100
Cell culture media (n=5)
101-116
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
103.9
217.33
887.52
102.82
203.34
879.17
Standard deviation
9.13
20.97
81.83
11.7
15.47
90.2
C V (%)
8.79
9.65
9.22
11.38
7.61
10.26
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CASP4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CASP4 in samples. No significant cross-reactivity or interference between Human CASP4 and analogues was observed.
