The Human Caspase-4 ELISA Kit is specially designed for the precise measurement of Caspase-4 levels in human serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit guarantees accurate and consistent results, making it the perfect choice for a variety of research purposes.Caspase-4 is a key enzyme in the inflammatory response pathway, playing a critical role in immune responses and cellular stress.
Dysregulation of Caspase-4 has been linked to various diseases including sepsis, inflammatory bowel disease, and neurodegenerative disorders, highlighting its importance as a biomarker for understanding and treating these conditions.Order the Human Caspase-4 ELISA Kit today and unlock new insights into the function and implications of Caspase-4 in health and disease.
Product Name:
Human CASP4 (Caspase 4) ELISA Kit
SKU:
HUES01819
Target:
Human CASP4 (Caspase 4)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CASP4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CASP4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CASP4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CASP4. You can calculate the concentration of Human CASP4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-106
92-104
94-108
Average (%)
97
97
99
1:4
Range (%)
86-100
83-94
83-95
Average (%)
93
89
88
1:8
Range (%)
88-101
83-93
87-100
Average (%)
95
88
92
1:16
Range (%)
92-108
85-98
85-95
Average (%)
100
91
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-97
91
EDTA plasma (n=5)
93-105
99
Cell culture media (n=5)
95-105
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.52
1.43
3.98
0.52
1.52
3.66
Standard deviation
0.03
0.07
0.18
0.03
0.08
0.11
C V (%)
5.77
4.9
4.52
5.77
5.26
3.01
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CASP4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CASP4 in samples. No significant cross-reactivity or interference between Human CASP4 and analogues was observed.
