The Human CASP8 (Caspase 8) CLIA Kit is specifically designed for the precise measurement of caspase 8 levels in human samples, including serum, plasma, and cell culture supernatants. With excellent sensitivity and specificity, this kit ensures accurate and dependable results, making it highly suitable for various research purposes.Caspase 8 is a key protein involved in programmed cell death, playing a vital role in various physiological processes and diseases.
Dysregulation of caspase 8 is associated with conditions such as cancer, autoimmune disorders, and neurodegenerative diseases, making it a valuable biomarker for investigating these diseases and developing potential treatments.Overall, the Human CASP8 CLIA Kit is an essential tool for researchers looking to study caspase 8 function and its implications in health and disease, providing reliable data for advanced scientific studies.
Product Name:
Human CASP8 (Caspase 8) CLIA Kit
SKU:
HUES00435
Target:
Human CASP8 (Caspase 8)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CASP8. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CASP8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CASP8, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CASP8. You can calculate the concentration of Human CASP8 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-118
85-97
85-100
Average (%)
110
91
91
1:4
Range (%)
89-102
91-103
86-98
Average (%)
94
98
92
1:8
Range (%)
93-105
87-102
88-101
Average (%)
99
93
94
1:16
Range (%)
86-97
85-99
84-98
Average (%)
91
91
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-117
108
EDTA plasma (n=5)
89-100
94
Cell culture media (n=5)
83-96
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
104.93
221.39
744.87
113.72
239.23
688.26
Standard deviation
13.49
18.31
83.13
11.35
19.33
65.66
C V (%)
12.86
8.27
11.16
9.98
8.08
9.54
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CASP8 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CASP8 in samples. No significant cross-reactivity or interference between Human CASP8 and analogues was observed.
