The Human Casp8 (Caspase 8) ELISA Kit is specifically developed for the precise quantification of caspase 8 levels in human samples including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and accuracy, this kit ensures consistent and trustworthy results, making it a valuable tool for various research applications.Caspase 8 is a key protein involved in apoptosis, playing a crucial role in regulating cell death and survival. Dysregulation of caspase 8 has been linked to various diseases, including cancer, autoimmune disorders, and infectious diseases.
As such, accurate measurement of caspase 8 levels can provide valuable insights into disease mechanisms and aid in the development of potential therapeutic interventions.The Human Casp8 ELISA Kit from AssayGenie offers researchers a reliable and efficient method for studying caspase 8 function and its implications in disease pathology. With its high specificity and sensitivity, this kit is a valuable asset for advancing research in the field of cell biology and molecular medicine.
Product Name:
Human CASP8 (Caspase 8) ELISA Kit
SKU:
HUES01818
Target:
Human CASP8 (Caspase 8)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CASP8. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CASP8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CASP8, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CASP8. You can calculate the concentration of Human CASP8 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
83-96
95-109
91-105
Average (%)
90
102
96
1:4
Range (%)
89-103
83-94
88-100
Average (%)
94
89
95
1:8
Range (%)
92-109
83-96
83-96
Average (%)
100
89
89
1:16
Range (%)
92-106
86-98
84-97
Average (%)
100
91
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-108
99
EDTA plasma (n=5)
90-104
95
Cell culture media (n=5)
88-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.55
1.21
4.89
0.53
1.13
4.48
Standard deviation
0.03
0.06
0.16
0.04
0.05
0.14
C V (%)
5.45
4.96
3.27
7.55
4.42
3.13
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CASP8 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CASP8 in samples. No significant cross-reactivity or interference between Human CASP8 and analogues was observed.
