The Human CAV-1 (Caveolin-1) ELISA Kit is specifically designed for the precise measurement of caveolin-1 levels in human samples including serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring accurate and consistent results that are perfect for a variety of research purposes. Caveolin-1 is a key protein involved in cellular signaling and lipid transport, playing a crucial role in various physiological processes.
Dysregulation of caveolin-1 has been linked to diseases such as cancer, diabetes, and cardiovascular disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions. The Human CAV-1 (Caveolin-1) ELISA Kit from Assay Genie provides researchers with a reliable tool to investigate caveolin-1 levels in human samples and unravel its role in health and disease. Order yours today and take your research to the next level.
Product Name:
Human Cav-1 (Caveolin-1) ELISA Kit
SKU:
HUES01829
Target:
Human Cav-1 (Caveolin-1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Cav-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Cav-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Cav-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Cav-1. You can calculate the concentration of Human Cav-1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
94-105
95-110
Average (%)
99
100
103
1:4
Range (%)
92-104
87-100
87-99
Average (%)
98
92
93
1:8
Range (%)
91-106
86-98
87-98
Average (%)
97
92
92
1:16
Range (%)
89-102
83-97
83-95
Average (%)
96
89
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-110
100
EDTA plasma (n=5)
84-97
91
Cell culture media (n=5)
95-110
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.99
2.68
6.73
1.0
2.82
6.9
Standard deviation
0.06
0.14
0.25
0.07
0.16
0.35
C V (%)
6.06
5.22
3.71
7.0
5.67
5.07
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human Cav-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Cav-1 in samples. No significant cross-reactivity or interference between Human Cav-1 and analogues was observed.
