Human CCL14 (Chemokine C-C-Motif Ligand 14) CLIA Kit
The Human CCL14 (Chemokine C-C Motif Ligand 14) CLIA Kit is specifically designed for the quantitative detection of CCL14 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reliable results for a variety of research applications.CCL14 is a chemokine protein that plays a crucial role in the regulation of inflammation and immune cell migration. It is involved in various inflammatory conditions such as asthma, atherosclerosis, and rheumatoid arthritis.
Studying CCL14 levels can provide valuable insights into the pathogenesis of these diseases and aid in the development of targeted therapies.The Human CCL14 CLIA Kit from Assay Genie is a valuable tool for researchers looking to investigate the role of CCL14 in inflammatory and immune-related disorders. Its user-friendly format and robust performance make it an essential addition to any research laboratory.
Product Name:
Human CCL14 (Chemokine C-C-Motif Ligand 14) CLIA Kit
SKU:
HUES00449
Target:
Human CCL14 (Chemokine C-C-Motif Ligand 14)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CCL14. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CCL14 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CCL14, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CCL14. You can calculate the concentration of Human CCL14 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
102-118
100-113
Average (%)
100
110
107
1:4
Range (%)
92-107
92-106
94-110
Average (%)
99
98
102
1:8
Range (%)
101-115
94-106
90-102
Average (%)
108
101
95
1:16
Range (%)
94-106
100-113
94-108
Average (%)
99
105
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
95
EDTA plasma (n=5)
89-102
95
Cell culture media (n=5)
86-100
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
39.77
127.62
317.92
38.63
118.11
324.41
Standard deviation
3.47
11.29
22.0
3.64
9.73
26.8
C V (%)
8.73
8.85
6.92
9.42
8.24
8.26
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CCL14 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CCL14 in samples. No significant cross-reactivity or interference between Human CCL14 and analogues was observed.
