Human CCL3L1 (Chemokine C-C-Motif Ligand 3 Like Protein 1) CLIA Kit
The Human CCL3L1 (Chemokine C-C Motif Ligand 3-Like Protein 1) CLIA Kit is a cutting-edge assay designed for the accurate detection of CCL3L1 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers unparalleled sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.CCL3L1 is a key chemokine involved in immune response and inflammation, playing a vital role in recruiting immune cells to sites of infection or injury.
Dysregulation of CCL3L1 has been implicated in various diseases such as autoimmune disorders, inflammatory conditions, and infectious diseases, highlighting its importance as a biomarker for studying these conditions and developing potential therapies.Overall, the Human CCL3L1 CLIA Kit provides researchers with a powerful tool for investigating the role of CCL3L1 in health and disease, offering a comprehensive solution for high-quality quantitative analysis.
Product Name:
Human CCL3L1 (Chemokine C-C-Motif Ligand 3 Like Protein 1) CLIA Kit
SKU:
HUES00450
Target:
Human CCL3L1 (Chemokine C-C-Motif Ligand 3 Like Protein 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CCL3L1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CCL3L1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CCL3L1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CCL3L1. You can calculate the concentration of Human CCL3L1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-99
91-105
100-114
Average (%)
93
99
108
1:4
Range (%)
89-102
98-113
89-103
Average (%)
96
106
94
1:8
Range (%)
93-108
85-99
95-111
Average (%)
101
91
103
1:16
Range (%)
92-107
88-101
95-109
Average (%)
98
94
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-110
103
EDTA plasma (n=5)
87-98
92
Cell culture media (n=5)
98-111
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.77
98.67
402.56
45.01
92.77
413.15
Standard deviation
4.15
10.19
44.89
4.58
8.12
43.46
C V (%)
8.34
10.33
11.15
10.18
8.75
10.52
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CCL3L1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CCL3L1 in samples. No significant cross-reactivity or interference between Human CCL3L1 and analogues was observed.
