Human CCL3L1 (Chemokine C-C-Motif Ligand 3 Like Protein 1) ELISA Kit
The Human CCL3L1 (Chemokine C-C motif ligand 3-like protein 1) ELISA Kit is specially designed for the precise measurement of CCL3L1 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and consistent results, making it suitable for a variety of research applications.CCL3L1 is a crucial chemokine protein involved in immune response and inflammation, playing a key role in regulating the migration and activation of immune cells.
Dysregulation of CCL3L1 has been implicated in various inflammatory diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.With the Human CCL3L1 ELISA Kit, researchers can accurately quantify CCL3L1 levels in biological samples, providing valuable insights into the role of this protein in health and disease. Trust in this kit for reliable and reproducible results in your research endeavors.
Product Name:
Human CCL3L1 (Chemokine C-C-Motif Ligand 3 Like Protein 1) ELISA Kit
SKU:
HUES01853
Target:
Human CCL3L1 (Chemokine C-C-Motif Ligand 3 Like Protein 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CCL3L1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CCL3L1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CCL3L1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CCL3L1. You can calculate the concentration of Human CCL3L1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-106
85-100
92-103
Average (%)
97
91
97
1:4
Range (%)
93-106
85-98
82-94
Average (%)
98
90
89
1:8
Range (%)
90-105
82-93
81-92
Average (%)
96
87
87
1:16
Range (%)
91-106
81-91
80-92
Average (%)
97
86
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-105
98
EDTA plasma (n=5)
87-98
93
Cell culture media (n=5)
85-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
230.19
696.41
2213.5
215.95
724.84
2270.93
Standard deviation
12.06
39.07
120.19
11.81
31.31
77.89
C V (%)
5.24
5.61
5.43
5.47
4.32
3.43
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CCL3L1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CCL3L1 in samples. No significant cross-reactivity or interference between Human CCL3L1 and analogues was observed.
