The Human CCND1 (Cyclin D1) CLIA Kit is specifically designed for the accurate and sensitive measurement of Cyclin D1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high specificity and precision, providing reliable results for various research applications.Cyclin D1 is a key regulator of the cell cycle, controlling the progression from G1 phase to S phase. Abnormalities in Cyclin D1 expression have been associated with various diseases, including cancer, making it a valuable biomarker for studying and understanding these conditions.
With its high sensitivity and ease of use, the Human CCND1 CLIA Kit is an essential tool for researchers investigating the role of Cyclin D1 in disease development and progression. Get accurate and reproducible results with this advanced kit from Assay Genie.
Product Name:
Human CCND1 (Cyclin-D1) CLIA Kit
SKU:
HUES01115
Target:
Human CCND1 (Cyclin-D1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CCND1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CCND1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CCND1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CCND1. You can calculate the concentration of Human CCND1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-110
102-121
97-113
Average (%)
103
110
103
1:4
Range (%)
86-102
97-114
90-101
Average (%)
93
104
96
1:8
Range (%)
97-112
103-116
90-104
Average (%)
104
109
96
1:16
Range (%)
88-104
84-98
91-104
Average (%)
95
91
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-109
103
EDTA plasma (n=5)
100-116
107
Cell culture media (n=5)
91-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
206.63
395.19
1925.03
223.05
356.93
1941.63
Standard deviation
17.34
32.52
118.58
20.54
28.45
146.98
C V (%)
8.39
8.23
6.16
9.21
7.97
7.57
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CCND1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CCND1 in samples. No significant cross-reactivity or interference between Human CCND1 and analogues was observed.
