Human CD276 (Cluster of Differentiation 276) CLIA Kit
The Human CD276 (Cluster of Differentiation 276) CLIA Kit is a highly sensitive and specific assay designed to detect CD276 levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.CD276, also known as B7-H3, is a cell surface protein that plays a critical role in immune regulation and cancer progression. Its expression has been linked to various cancers, making it a potential biomarker for cancer diagnosis and treatment monitoring.
Additionally, CD276 is involved in T-cell activation and immune response modulation, making it a valuable target for immunotherapy development.Overall, the Human CD276 CLIA Kit offers researchers a powerful tool for studying the role of CD276 in cancer, immune response, and other diseases, potentially leading to the development of new therapeutic strategies.
Product Name:
Human CD276 (Cluster of Differentiation 276) CLIA Kit
SKU:
HUES00843
Target:
Human CD276 (Cluster of Differentiation 276)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CD276. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CD276 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CD276, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CD276. You can calculate the concentration of Human CD276 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-116
89-102
94-107
Average (%)
108
95
101
1:4
Range (%)
91-107
90-103
95-109
Average (%)
98
96
101
1:8
Range (%)
95-106
91-102
93-109
Average (%)
100
96
101
1:16
Range (%)
102-114
96-112
94-106
Average (%)
108
102
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-109
99
EDTA plasma (n=5)
92-104
98
Cell culture media (n=5)
99-112
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.51
1.06
3.88
0.49
1.12
3.86
Standard deviation
0.04
0.12
0.28
0.06
0.09
0.25
C V (%)
7.84
11.32
7.22
12.24
8.04
6.48
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CD276 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CD276 in samples. No significant cross-reactivity or interference between Human CD276 and analogues was observed.
