Human CD4 (Cluster of Differentiation 4) ELISA Kit
The Human CD4 (Cluster of Differentiation 4) ELISA Kit is specifically designed for the accurate detection of CD4 levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.CD4 is a key protein found on the surface of T-helper cells, playing a crucial role in the immune response and immune system regulation.
Monitoring CD4 levels is essential in studying immune function, HIV progression, and autoimmune diseases. This ELISA kit provides researchers with a valuable tool for investigating the role of CD4 in various diseases and for developing potential therapeutic interventions.
Product Name:
Human CD4 (Cluster of Differentiation 4) ELISA Kit
SKU:
HUES01896
Target:
Human CD4 (Cluster of Differentiation 4)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CD4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CD4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CD4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CD4. You can calculate the concentration of Human CD4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-110
86-99
88-102
Average (%)
102
91
95
1:4
Range (%)
90-103
88-98
85-101
Average (%)
96
93
92
1:8
Range (%)
89-101
86-96
88-101
Average (%)
95
91
93
1:16
Range (%)
93-110
83-95
87-102
Average (%)
100
90
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
100
EDTA plasma (n=5)
90-107
97
Cell culture media (n=5)
91-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.99
1.69
9.32
0.94
1.81
8.91
Standard deviation
0.06
0.08
0.31
0.05
0.09
0.37
C V (%)
6.06
4.73
3.33
5.32
4.97
4.15
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CD4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CD4 in samples. No significant cross-reactivity or interference between Human CD4 and analogues was observed.
