Human CD42 (Cluster of Differentiation 42) CLIA Kit
The Human CD42 (Cluster of Differentiation 42) CLIA Kit is a powerful tool for the precise quantification of CD42 levels in human samples. This kit offers high sensitivity and specificity, allowing for accurate and consistent results across a variety of research settings.CD42, also known as glycoprotein Ib alpha, is a crucial component of the platelet glycoprotein Ib-IX-V complex, which plays a key role in platelet adhesion and aggregation.
Dysregulation of CD42 expression or function has been linked to various hematological disorders, making it a valuable biomarker for studying these conditions.With its ease of use and reliability, the Human CD42 CLIA Kit is an essential tool for researchers exploring the role of CD42 in health and disease. Get your kit today and unlock the potential of CD42 research in your lab.
Product Name:
Human CD42 (Cluster of Differentiation 42) CLIA Kit
SKU:
HUES00136
Target:
Human CD42 (Cluster of Differentiation 42)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CD42. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CD42 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CD42, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CD42. You can calculate the concentration of Human CD42 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-111
102-118
88-104
Average (%)
103
109
95
1:4
Range (%)
100-115
95-108
96-110
Average (%)
108
103
102
1:8
Range (%)
95-110
100-117
96-112
Average (%)
102
108
102
1:16
Range (%)
101-116
100-117
101-117
Average (%)
107
107
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-105
97
EDTA plasma (n=5)
97-109
102
Cell culture media (n=5)
87-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
261.28
507.74
1695.37
274.75
528.05
1633.08
Standard deviation
32.97
53.16
157.67
30.55
38.28
135.38
C V (%)
12.62
10.47
9.3
11.12
7.25
8.29
Sample type &Sample volume:
Plasma; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CD42 concentrations in Plasma.
Specificity:
This kit recognizes Human CD42 in samples. No significant cross-reactivity or interference between Human CD42 and analogues was observed.
