The Human CD59 (Protectin) CLIA Kit is specifically tailored for the precise quantification of CD59 levels in human samples including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit ensures accurate and consistent results, making it perfect for a variety of research purposes.CD59, also known as protectin, is a critical regulator of the complement system, a part of the immune system responsible for defending the body against infections.
Dysregulation of CD59 has been linked to various diseases such as autoimmune disorders, hemolytic anemia, and myocardial infarction. Therefore, measuring CD59 levels can provide valuable insights into the pathophysiology of these conditions and aid in the development of targeted therapeutic interventions.Get reliable and actionable data with the Human CD59 (Protectin) CLIA Kit, a powerful tool for unraveling the mysteries of immune system dysfunction and unlocking new treatment avenues.
Product Name:
Human CD59 (Protectin) CLIA Kit
SKU:
HUES00444
Target:
Human CD59 (Protectin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CD59. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CD59 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CD59, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CD59. You can calculate the concentration of Human CD59 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-103
92-110
89-103
Average (%)
95
100
95
1:4
Range (%)
89-101
89-101
94-107
Average (%)
96
96
99
1:8
Range (%)
93-107
87-99
95-107
Average (%)
99
93
102
1:16
Range (%)
91-108
83-95
84-99
Average (%)
99
90
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-117
108
EDTA plasma (n=5)
96-111
104
Cell culture media (n=5)
100-117
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.6
120.88
475.96
54.1
109.3
441.4
Standard deviation
5.4
13.2
50.02
5.46
10.83
48.29
C V (%)
9.89
10.92
10.51
10.09
9.91
10.94
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CD59 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CD59 in samples. No significant cross-reactivity or interference between Human CD59 and analogues was observed.
